目的 探讨白芥子散及其拆方对过敏性哮喘大鼠施药局部皮肤和血清中免疫球蛋白(Ig)-E、白介素(IL)-4、干扰素(IFN)-γ与肿瘤坏死因子(TNF)-α的影响。方法 将80只SD大鼠随机分成正常对照组、模型对照组、阳性对照组、白芥子散穴位组、白芥子散非穴位组、白芥子穴位组、延胡索穴位组、甘遂穴位组、细辛穴位组和生姜穴位组,每组8只。除正常对照组外,其余9组均采用卵清蛋白(OVA)致敏法建立过敏性哮喘模型。HE染色法观察肺组织的形态变化;ELISA法检测施药局部皮肤和血清中Ig-E、IL-4、IFN-γ及TNF-α的水平。结果 白芥子散穴位组肺泡结构基本正常且炎性细胞浸润程度明显减轻,与正常对照组较为相似;各治疗穴位组均可使血清中Ig-E、IL-4、IFN-γ及TNF-α水平有所回调,尤以白芥子散穴位组、白芥子穴位组和细辛穴位组最佳(P<0.01);白芥子散穴位组、白芥子穴位组与细辛穴位组施药局部皮肤中Ig-E水平低于正常值。结论 白芥子散可有效治疗过敏性哮喘,能够显著地增强IFN-γ并减弱Ig-E、IL-4和TNF-α在施药局部皮肤及血清中的表达,且施药局部皮肤中各指标值的回调强度均高于血清。各拆方药味中,白芥子和细辛对Ig-E、TNF-α及IFN-γ的作用较强,而延胡索主要对IL-4产生作用。
Abstract
OBJECTIVE To investigate the effects of baijiezi powder and its disassembled formula on the immunoglobulin (Ig)-E, interleukin (IL)-4, interferon (IFN)-γ and tumor necrosis factor (TNF)-α in local skin of applied drugs and lung tissues of rats with allergic asthma. METHODS Eighty rats were randomly divided into normal control group, model control group, positive control group, baijiezi powder acupoint group, baijiezi powder non-acupoint group, Semen Sinapis Albae acupoint group, Corydalis Rhizoma acupoint group, Radix Kansui acupoint group, asarum acupoint group and Ginger acupoint group, 8 in each group. In addition to the blank control group, the other nine groups were sensitized by ovalbumin (OVA) to establish a model of allergic asthma. The morphological changes of lung tissue were observed by HE staining. The contents of the Ig-E, IL-4, IFN-γ and TNF-α in local skin of application and serum were detected by enzyme-linked immunosorbent assay (ELISA). RESULTS The infiltration degree of inflammatory cells was obviously reduced and the alveolar structure was basically normal in baijiezi powder acupoint group, which was similar to the normal control group. The levels of the Ig-E, IL-4, IFN-γ and TNF-α in serum can be recalled in each treatment acupoint group. Especially,baijiezi powder acupoint group, Semen Sinapis Albae acupoint group and asarum acupoint group were the best (P<0.01). The level of Ig-E in the local skin for the drug application of baijiezi powder acupoint group, Semen Sinapis Albae acupoint group and asarum acupoint group was lower than the normal value. CONCLUSION Baijiezi powder can effectively treat allergic asthma, can signifcantly enhance the IFN-γ and weaken the expression of Ig-E, IL-4 and TNF-α in the local skin of the application and serum. Meanwhile, the correction intensity of each index value in local skin of applied drugs of baijiezi powder was higher than that of serum. Among all the disassembled herbs, Semen Sinapis Albae and asarum have a strong effect on Ig-E, TNF-α and IFN-γ, while Corydalis Rhizoma mainly has an effect on IL-4.
关键词
白芥子散 /
穴位贴敷 /
过敏性哮喘 /
免疫球蛋白-E /
白介素-4 /
干扰素-γ /
肿瘤坏死因子-α
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Key words
baijiezi powder /
acupoint application /
allergic asthma /
immunoglobulin-E /
interleukin-4 /
interferon-γ /
tumor necrosis factor-α
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中图分类号：
R965
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脚注
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基金
国家自然科学基金青年基金项目资助(81703944);黑龙江中医药大学博士创新基金项目资助(2017bs10);黑龙江中医药大学“优秀创新人才支持计划”项目资助(2018年);国家自然科学基金项目资助(81473359);黑龙江省科技厅青年基金项目资助(QC07C107);黑龙江省教育厅科学技术研究项目资助(11531357);哈尔滨市应用技术研究与开发项目资助(RAXXJ047);黑龙江省自然科学基金优秀青年项目资助(YQ2019H031)
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